https://doi.org/10.1101/2023.10.01.560393
Видання: 2023
Видавець: Cold Spring Harbor Laboratory
Автори:
- Eric I. Nayman
- Brooke A. Schwartz
- Michaela Polmann
- Alayna C. Gumabong
- Max Nieuwdorp
- Trevor Cickovski
- Kalai Mathee
Анотація
Abstract<jats:sec id="s21">PurposeDecreased gut microbiota production of short-chain fatty acids (SCFAs) has been implicated in type 2 diabetes mellitus (T2DM) disease progression. Most microbiome studies focus on ethnic majorities. This study aims to understand microbiome differences between an ethnic majority (the Dutch) and minority (the South-Asian Surinamese (SAS)) group with a lower and higher prevalence of T2DM, respectively.</jats:sec><jats:sec id="s22">MethodsMicrobiome data from the Healthy Life in an Urban Setting (HELIUS) cohort were used. The 16S rRNA V4 region was sequenced. Two age– and gender-matched groups were compared: the Dutch (n = 41) and SAS (n = 43). Microbial compositions were generated via DADA2. Alpha and beta-diversity and Principal Coordinate Analysis (PCoA) were computed. DESeq2 differential bacterial abundance and LEfSe biomarker analyses were performed to determine discriminating features. Co-occurrence networks were constructed to examine gut ecology.</jats:sec><jats:sec id="s23">ResultsA tight cluster of bacterial abundances was observed in the Dutch women, which overlapped with some of the SAS microbiomes. The Dutch gut contained a more interconnected microbial ecology, whereas the SAS network was dispersed.Bacteroides caccae, Butyricicoccus, Alistipes putredinis, Coprococcus comes,Odoribacter splanchnicus,andLachnospiracharacterized the Dutch gut.Haemophilus,Bifidobacterium,andAnaerostipes hadruscharacterized the SAS gut. All butLachnospiraand certain strains ofHaemophilusare known SCFA producers.</jats:sec><jats:sec id="s24">ConclusionThe Dutch gut microbiome was distinguished from the SAS by diverse, differentially abundant SCFA-producing taxa with significant cooperation. The dynamic ecology observed in the Dutch was lost in the SAS. The higher prevalence of T2DM in the SAS may be associated with the dysbiosis observed.</jats:sec>
Список літератури
- Münger E , Montiel-Castro AJ , Langhans W , Pacheco-López G . Reciprocal Interactions Between Gut Microbiota and Host Social Behavior. Frontiers in Integrative Neuroscience 2018;12.
https://doi.org/10.3389/fnint.2018.00021 - National Diabetes Statistics Report | Diabetes | CDC 2022. https://www.cdc.gov/diabetes/data/statistics-report/index.html (accessed September 7, 2023).
- International Diabetes Federation (2019) IDF Diabetes Atlas: Ninth edition 2019. Available from https://diabetesatlas.org/upload/resources/material/20200302_133351_IDFATLAS9e-final-web.pdf. Accessed Sept 7th, 2023., n.d.
- Letchumanan G , Abdullah N , Marlini M , Baharom N , Lawley B , Omar MR , et al. Gut Microbiota Composition in Prediabetes and Newly Diagnosed Type 2 Diabetes: A Systematic Review of Observational Studies. Frontiers in Cellular and Infection Microbiology 2022;12.
https://doi.org/10.3389/fcimb.2022.943427 - Willis AD. Rarefaction , Alpha Diversity , and Statistics. Frontiers in Microbiology 2019;10.
https://doi.org/10.3389/fmicb.2019.02407 - Hu C , Niu X , Chen S , Wen J , Bao M , Mohyuddin SG , et al. A Comprehensive Analysis of the Colonic Flora Diversity, Short Chain Fatty Acid Metabolism, Transcripts, and Biochemical Indexes in Heat-Stressed Pigs. Frontiers in Immunology 2021;12.
https://doi.org/10.3389/fimmu.2021.717723 - Amiri P , Hosseini SA , Ghaffari S , Tutunchi H , Ghaffari S , Mosharkesh E , et al. Role of Butyrate, a Gut Microbiota Derived Metabolite, in Cardiovascular Diseases: A comprehensive narrative review. Frontiers in Pharmacology 2022;12.
https://doi.org/10.3389/fphar.2021.837509 - Nogal A , Louca P , Zhang X , Wells PM , Steves CJ , Spector TD , et al. Circulating Levels of the Short-Chain Fatty Acid Acetate Mediate the Effect of the Gut Microbiome on Visceral Fat. Frontiers in Microbiology 2021;12.
https://doi.org/10.3389/fmicb.2021.711359 - Hiippala K , Barreto G , Burrello C , Diaz-Basabe A , Suutarinen M , Kainulainen V , et al. Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro. Frontiers in Microbiology 2020;11.
https://doi.org/10.3389/fmicb.2020.575455 - [A new butyric acid-producing bacteroides species: B. splanchnicus n. sp, author’s transl)]. Zentralbl Bakteriol Orig A, № 231, с. 133
- Parker BJ , Wearsch PA , Veloo ACM , Rodriguez-Palacios A . The Genus Alistipes: Gut Bacteria With Emerging Implications to Inflammation, Cancer, and Mental Health. Frontiers in Immunology 2020;11.
https://doi.org/10.3389/fimmu.2020.00906 - ANOVA-Like Differential Expression (ALDEx) Analysis for Mixed Population RNA-Seq | PLOS ONE n.d. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067019 (accessed September 13, 2023).
- Chen J , Qin Q , Yan S , Yang Y , Yan H , Li T , et al. Gut Microbiome Alterations in Patients With Carotid Atherosclerosis. Frontiers in Cardiovascular Medicine 2021;8.
https://doi.org/10.3389/fcvm.2021.739093 - Li Y , Kang Y , Du Y , Chen M , Guo L , Huang X , et al. Effects of Konjaku Flour on the Gut Microbiota of Obese Patients. Frontiers in Cellular and Infection Microbiology 2022;12.
https://doi.org/10.3389/fcimb.2022.771748
Дані публікації
| Кількість цитувань | 0 |
| Кількість джерел у списку літератури: | 14 |
| Видання індексується в Scopus | Ні |
| Видання індексується в Web of Science | Ні |
